BKS DB Mouse Brain Microvascular Endothelial Cells
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quantity
Produktdetaljer
Katalognummer: 870 - ABC-TC5475
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 1 vial
Parameters
| Additional information | For research use only |
|---|---|
| Storage and shipping | Liquid Nitrogen |
ABC-TC5475
BKS DB Mouse Brain Microvascular Endothelial Cells
BKS db Mouse Brain Microvascular Endothelial Cells are isolated from the brain microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
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ABC-TC5485
BKS DB Mouse Lung Microvascular Endothelial Cells
BKS db Mouse Lung Microvascular Endothelial Cells are isolated from the Lung Microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Lung Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
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ABC-TC5480
BKS DB Mouse Dermal Microvascular Endothelial Cells
BKS db Mouse Dermal Microvascular Endothelial Cells are isolated from the dermal microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Dermal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
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ABC-TC3524
Human Brain Microvascular Endothelial Cells
Human Brain Microvascular Endothelial Cells were initiated by elutriation of dispase dissociated normal human brain cortex tissue.These cells were originated using Complete Serum-Free Medium Kit, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells.
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ABC-TC4028
Porcine Brain Microvascular Endothelial Cells
Porcine Brain Microvascular Endothelial Cells are derived from healthly porcinine Brain tissue. Porcine Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Porcine Brain Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC4096
Rat brain microvascular endothelial cells
Rat primary brain microvascular endothelial cells (RPBECs) are isolated from tissue of 1-day-old neonatal Sprague-Dawley rats. RPBECs are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.
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