NF-κB Reporter Cellular Assay Pack (HCT116)
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Katalognummer: 421 - 79326
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 2 vials
79325
NF-κB Reporter Cellular Assay Pack (CHOK1)
The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- CHO-K1 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine. _x000D_Additionally, the pack includes cell culture medium (Thaw Medium 3) that has been optimized for use with CHO-K1 cells. Thaw Medium 3 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.
2017.5 €
79327
NF-κB Reporter Cellular Assay Pack (HEK293)
The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)-HEK293 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 1) that has been optimized for use with HEK293 cells. Thaw Medium 1 includes 10% fetal bovine serum, non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_
2422.5 €
60623
NF-κB Reporter (Luc) - HCT116 Cell Line
NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The<br />firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of<br />the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors<br />bind to the DNA response elements to induce transcription of the luciferase gene._x000D_The NF-κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling<br />in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated<br />molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern<br />(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for<br />establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.<br />This cell line can be further modified to allow investigation of downstream NF-κB activities as a<br />result of targeted genetic mutation(s).
1792.5 €
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