
Katalognummer: 855 - ELK4762
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 1 plate of 96 wells
| Additional information | The microtiter plate provided in this kit has been pre-coated with an antibody specific to Divalent Metal Transporter 1 (DMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Divalent Metal Transporter 1 (DMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Divalent Metal Transporter 1 (DMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Divalent Metal Transporter 1 (DMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
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| Storage and shipping | The Human DMT1 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C. |

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