
Katalognummer: 855 - ELK7037
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 1 plate of 96 wells
| Additional information | The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2?). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2?). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 2 Alpha (HIF2?), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 2 Alpha (HIF2?) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
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| Storage and shipping | The Rat HIF2a ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C. |

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