HighQC™ Cord Blood Mononuclear Cells AcceGen

HighQC™ Cord Blood Mononuclear Cells

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Produktdetaljer

Katalognummer: 870 - ABC-SC0128T

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

AcceGenGentaur

Storlek: 1 vial

Parameters

Storage and shipping	Liquid Nitrogen

ABC-SC0128T

HighQC™ Cord Blood Mononuclear Cells

Human cord blood mononuclear cells are separated from umbilical cord blood by means of a density gradient centrifugation protocol. MNCs are screened for the expression CD45 and checked for viability using flow cytometry prior to cryopreservation.

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HighQC™ Human Mobilized Peripheral Blood Mononuclear Cells

Human Peripheral Blood Mononuclear Cells (PBMC) are blood cells with a single round nucleus. These cells include lymphocytes (T, B and NK cells), monocytes and dendritic cells. Mononuclear cells are parts of the immune system which is critical for cell-mediated and humoral immunity. Mobilized peripheral blood is collected after the donor is treated with mobilization agent such as G-CSF. Such treatment mobilizes blood stem cells that are normally reside in the bone marrow to enter the blood stream. The content of CD34+ stem cells in the blood is therefore the most important parameter of graft quality. The optimal CD34+ cell dose is widely accepted to be 5 × 106/kg. Particularly, the more primitive CD34+/CD38-subset has been shown to have high self-renewal and repopulation capability. Stem cell mobilization also affects the properties of other components of the graft such as T lymphocytes and NK cells. Gentaur Human Mobilized PBMCs are isolated from healthy donors that have been mobilized with GCSF collected in acid-citrate-dextrose formula A (ACDA) as an anticoagulant. All donors must be tested negative for HBV, HCV and HIV and are IRB consented.

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HighQC™ Human IPSC From Cord Blood Cells CD34+

iPSC lines are derived using a standardized integration-free, episomal reprogramming protocols from CD34+ cord blood cells.

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HighQC™ Nephron Progenitor Cells

Primary nephron progenitor cells from embryonic mouse kidney. The nephron progenitor expansion medium (NPEM) is a proprietary cocktail specifically developed for the propagation of primary nephron progenitor cells isolated from embryonic mouse kidneys.Highlights: Primary CITED1+ cells from the cap mesenchyme of the developing mouse kidneyHarvested from embryonic day 17.5 kidneys from mice of the ICR outbred stockMaintain progenitor characteristics and potential for differentiation up to 6 passages if propagated in NPEMNephron is the basic structural and functional unit of the kidneys. It is the structure that produces urine by a process that removes waste and excess substances by filtering blood. The nephron also regulates blood volume and blood pressure, as well as controls electrolytes and metabolites and regulates blood pH which make them vital to human life.

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HighQC™ RFP-Human Umbilical Cord-Derived Mesenchymal Stem Cell

HUcMSCs are isolated from umbilical cord matrix (Wharton's Jelly) and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HUcMSCs are puromycin resistant HUcMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HUcMSCs is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)), when cultured following the detailed protocol described below.

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HighQC™ Human Mobilized Peripheral Blood CD34+ Stem/Progenitor Cells; G-CSF And CXCR4 Antagonists

CD34 is most commonly associated with expression on hematopoietic progenitor cells found in bone marrow and cord blood. While compromising only 1.5% of bone marrow mononuclear cell population, CD34 stem cells are multipotent and responsible for all lymphohematopoietic lineages. These cells are now commonly used clinically as they have been shown to provide long-term durable donor-derived host lymphohematopoietic reconstitution.Donors are injected with a combination of CXCR4 antagonists and G-CSF, which stimulates the bone marrow to produce a large number of hematopoietic and progenitor stem cells and mobilizes them into the peripheral blood stream.CD34+ cells are isolated using positive immunomagnetic cell separation procedures from a mobilized leukapheresis collection.

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