ATAD2A Inhibitor Screening Assay Kit BPS Bioscience

ATAD2A Inhibitor Screening Assay Kit

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Katalognummer: 421 - 32601

Produktkategori: Företag och industri > Vetenskap och laboratorium

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The ATAD2A TR-FRET Assay Kit is designed to measure the inhibition_x000D_of ATAD2A binding to its substrate in a homogeneous 384 reaction format. This FRETbased_x000D_assay requires no time-consuming washing steps, making it especially suitable for_x000D_high throughput screening applications. The assay procedure is straightforward and_x000D_simple; a sample containing terbium-labeled donor, dye-labeled acceptor, ATAD2A,_x000D_substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence_x000D_intensity is measured using a fluorescence reader.

915 €

32605

ATAD2B Inhibitor Screening Assay Kit

The ATAD2B Inhibitor Screening Assay Kit is designed to measure the inhibition of ATAD2B binding to its substrate. The key to the kit is the specific binding of the ATAD2B bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing ATAD2B and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

1687.5 €

31109

ATAD2A, His-tag

Human Bromodomain ATAD2A, also known as ANCCA and ATPase Family AAA Domain-containing Protein 2, GenBank Accession No. NM_014109, a.a. 981 - 1108 corresponding to single bromodomain with N-terminal His-tag, MW = 16.2 kDa, expressed in an E. coli expression system.

607.5 €

82210

COX2 Inhibitor Screening Assay Kit

The COX2 Inhibitor Screening Assay Kit is designed to measure COX2 (cyclooxygenase 2) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant COX2, Amplex™ Red, Arachidonic Diluent, COX Assay Buffer, and 100% ethanol for 100 enzyme reactions.

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82516

Diablo:XIAP Inhibitor Screening Assay Kit

The Diablo:XIAP Inhibitor Screening Assay Kit is an ELISA-based assay designed to measure the binding between Diablo (also named Smac) and E3 ubiquitin-protein ligase XIAP (X-linked inhibitor of apoptosis, also known as mammalian IAP homolog A) for screening and profiling applications. The Diablo:XIAP Inhibitor Screening Assay Kit comes with enough purified Diablo (amino acids 56-239) and XIAP proteins, primary and secondary antibody, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. Diablo:XIAP Binding Chemiluminescent Assay Kit schematic.A 96-well plate is coated with purified Diablo protein. After coating and blocking, XIAP is added in an optimized assay buffer. Next, unbound XIAP is washed away and the plate is incubated with a primary antibody, followed by a secondary HRP-conjugated antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the binding of XIAP to Diablo.Need us to run inhibitor screens or profile your compounds against Diablo:XIAP binding? Check out ourUbiquitination Screening and Profiling Service.

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82726-1

USP9X Inhibitor Screening Assay Kit

The USP9X Inhibitor Screening Assay Kit is a fluorogenic assay kit designed to measure the activity of the deubiquitinating (DUB) enzyme USP9X (ubiquitin specific peptidase 9 X-linked) for screenin82726-1g and profiling applications. The kit contains enough purified USP9X, Ubiquitinated-AMC substrate, and assay buffer for 96 reactions.To determine the effect of an inhibitor on USP9X activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of the DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases, with a C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form, the energy emitted from fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with an λexcitation/λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP14? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

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