
Katalognummer: 855 - ELK5768
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 1 plate of 96 wells
| Additional information | The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to IL12A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated monoclonal antibody specific to IL27B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL35 in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
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| Storage and shipping | The Mouse IL35 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C. |

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