ION Vital - Viability Kit, live or dead assay kit for mammalian cells

5100

ION Vital - MitoVolt, mitochondrial membrane potential assay kit with JC-10

Mitochondrial membrane potential assay kit that uses JC-10. Compatible with fluorescence microscopy, flow cytometry, and plate reader assays.Healthy: Ex 540 nm / Em 590 nm | Compromised: Ex 490 nm / Em 525 nmION Vital - MitoVolt assay kit is the ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena. MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, the MitoVolt kit provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application.JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence. JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.

261.8 €

K502-100

Live/Dead Cell Viability Assay Kit (for Mammalian Cells)

972.6 €

1497527

ION Essentials Ratiometric Calcium - No wash ratiometric calcium assay kit

No wash, ratiometric calcium flux assay, 2 plates. Total assay solution compatible with plate reader and fluorescence microscopy applications. Excitation: 340, 380 nm | Emission: 505 nm | Kd: 145 nMRatiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.

144.5 €

2013C

ING-2 TMA+ Salt - yellow-green fluorescent sodium indicator

ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na⁺) indicator. Formerly known as Asante Natrium Green - 2 (ANG-2). Has improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na⁺ indicator for high-throughput screening applications targeting Na⁺ channels, and non-selective monovalent cation channels. Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.Excitation: 525 nm | Emission: 545 nm | Kd: 20 mMING-2 TMA⁺ salt is a membrane impermeable form of ING-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

283.9 €

2013F

ING-2 TMA+ Salt - yellow-green fluorescent sodium indicator

ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na⁺) indicator. Formerly known as Asante Natrium Green - 2 (ANG-2). Has improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na⁺ indicator for high-throughput screening applications targeting Na⁺ channels, and non-selective monovalent cation channels. Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.Excitation: 525 nm | Emission: 545 nm | Kd: 20 mMING-2 TMA⁺ salt is a membrane impermeable form of ING-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

283.9 €

2015C

ING-1 AM - yellow-green fluorescent sodium indicator

ION Natrium Green – 1 (ING-1) AM is a membrane permeable, yellow-green fluorescent, intracellular sodium (Na⁺) indicator. Formerly known as Asante Natrium Green - 1 (ANG-1). Has improved cellular loading and significantly higher brightness than SBFI. It has a lower affinity for sodium than ING-2.Excitation: 525 nm | Emission: 545 nm | Kd: 20 mM

332.35 €