NFAT Luciferase-RFP Reporter Lentivirus

78556

TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line

This cell line is a knockout of TCR (T Cell Receptor). The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells stably expressing the firefly luciferase gene under the control of NFAT response elements.This cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to parental NFAT-Luciferase Reporter Jurkat cells (BPS Bioscience #60621).

18457.5 €

79750

LILRB4 NFAT-Luciferase Reporter Jurkat Recombinant Cell Line

Recombinant Jurkat cells constitutively expressing human LILRB4 (Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 4, GenBank Accession #NM_001278426) and the firefly luciferase gene under the control of NFAT response elements.

14955 €

T6125

NFAT-Luciferase Stable RAW264.7 Cell Line

6041 €

79580

CRE/CREB Luciferase Reporter Lentivirus

The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. Upon phosphorylation, CREB forms a functionally active dimer that binds the CRE element within the promoters of target genes and activates transcription. CRE is at the focus of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms.The CRE/CREB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized cAMP response element (CRE) located upstream of the minimal TATA promoter. After transduction, activation of the cAMP/PKA signaling pathway in the target cells can be monitored by measuring the luciferase activity.

1402.5 €

78664

EGR1 Promoter Luciferase Reporter Lentivirus

The Early growth response 1 (EGR1, also known as ZNF268 or NGFi-A) Promoter Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human EGR1 promoter (~1.3 kb, Figure 1) . After transduction, activation of the EGR1 promoter in the target cells can be monitored by measuring the luciferase activity.Figure 1. Schematic of the lenti-vector used to generate the EGR1 Promoter Luciferase Reporter Lentivirus.

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79762

PD-1 (Mouse) / NFAT - Reporter - Jurkat Recombinant Cell Line

Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of mouse PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_008798).

16792.5 €