TurboTEV Protease, Recombinant

50308

TurboTEV Protease

TurboTEV Protease contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TurboTEV Protease is a restriction grade protease that has a robust activity at 4°C with high specificity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. TurboTEV Protease is a 52 kDa protein with both GST and His-tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag. In practice, 1 mg (10,000 units) of TurboTEV Protease cleaves >90% of 100 mg of a control target protein at 4°C in 16 hours. No non-specific cleavage has been observed under the same condition when TurboTEV Protease and the control target protein was mixed at 1:10 ratio.

922.5 €

9205-10

TurboTEV Protease, Recombinant

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9206-1

Turbo3C (HRV3C) Protease, Recombinant

992.4 €

160

Turbo Prime-olate™

424.4 €

170

Turbo Prime-olate™

343.4 €

50310

Turbonuclease

Ultra-pure version of Benzonase. Nonspecific endonuclease, hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length. MW = 27 kDa (homodimer). Expressed in E. coli. No detectable protease activity._x000D_TurboNuclease can be used to ;• reduce viscosity of cell lysates;• reduce back pressure of column loading;• remove nucleic acid contamination from sample preparations;• reduce nucleic acid contamination of Ni column purification;• reduce smearing in SDS-PAGE when used with 10%SDS or Gel Loading Dye; • make whole cell lysates;• reduce or prevent clumping of concentrated cells and thawed cells;• replace crude DNase I in many applications

682.5 €