BKS DB Mouse Aortic Endothelial Cells AcceGen

BKS DB Mouse Aortic Endothelial Cells

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Produktdetaljer

Katalognummer: 870 - ABC-TC5471

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

AcceGenGentaur

Storlek: 1 vial

Parameters

Additional information	For research use only
Storage and shipping	Liquid Nitrogen

ABC-TC5471

BKS DB Mouse Aortic Endothelial Cells

BKS db Mouse Aortic Endothelial Cells are isolated from the aorta of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Aortic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

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ABC-TC5499

BKS DB Mouse Vein Endothelial Cells

BKS db Mouse Vein Endothelial Cells are isolated from the vein of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

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ABC-TC5472

BKS DB Mouse Artery Endothelial Cells

BKS db Mouse Artery Endothelial Cells are isolated from the artery of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

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ABC-TC3493

Human Aortic Endothelial Cells

Primary Human Aortic Endothelial Cells were initiated from normal human descending aorta (aortic arch to renal artery).These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

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ABC-TC3902

Monkey Aortic Endothelial Cells

Monkey Aortic Endothelial Cells from Gentaur are isolated from aortic tissue of Cynomolgus Monkey. Monkey Aortic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Monkey Aortic Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC4025

Porcine Aortic Endothelial Cells

Porcine large artery endothelial cells are derived from healthly, plaque free porcine. PAOEC and PPAEC are cryopreserved at first passage and can be cultured and propagated at least 16 population doublings.

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