Human Bladder Epithelial Cells

ABC-TC4367

Human Bladder Epithelial Cells

Human Primary Bladder Epithelial Cells from Gentaur are isolated from normal human bladder tissue. Human Primary Bladder Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaurandrsquo; Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

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ALHE36

Human Bladder Epithelial Cells

1180 €

2-96007

Human Bladder PrimaCell™1: Normal Bladder Epithelial Cells

2022.75 €

ABC-TC3834

Human Tonsil Epithelial Cells

Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of actively differentiating epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium. The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues. In Situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial culture in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ. HTonEpiC from Gentaur Research Laboratories are isolated from human normal tonsil tissue. HTonEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HTonEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HTonEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTonEpiC are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.

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ABC-TC3515

Human Bladder Stromal Fibroblasts

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of the easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechanical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. As cells of mesenchymal origin, human bladder stromal fibroblasts (HBdSF) also secrete FGF-7, which interact primarily with bladder epithelial cells. FGF-7 secreted by HBdSF is essential for stimulating the formation of a multilayered stratified bladder epithelium. HBdSF from Gentaur Research Laboratories are isolated from human bladder tissue. HBdSF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBdSF are characterized by immunofluorescent method with antibody to fibronectin. HBdSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

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ABC-HP021X

Human Stomach Epithelial Cells

Human Stomach Epithelial Cells are isolated from normal human stomach tissue. Cells are cryopreserved at passage 3 and delivered frozen.

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