Human Colonic Smooth Muscle Cells

ABC-TC3572

Human Colonic Smooth Muscle Cells

Smooth muscle contraction is the fundamental event in gastrointestinal motion. Inflammation of the human intestine causes thickening of the smooth muscle layers which results from the increases in the smooth muscle-specific actins. The increased smooth muscle actins may affect force production and further demonstrate the plasticity of smooth muscle in the inflamed intestine. Human intestinal smooth muscle cells respond to IL-1-beta and TNF-alpha stimulation by releasing IL-6, which might significantly contribute to the overall systemic inflammatory response. The colonic smooth muscle cells possess a different subtype of somatostatin receptor from gastric smooth muscle cells. The availability of human gastrointestinal smooth muscle cells in culture will considerably enhance our ability to study the contractile, proliferative and connective tissue responses of the smooth muscle of the human gastrointestinal tract.HCSMC from Gentaur Research Laboratories are isolated from the human colon. HCSMC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCSMC are characterized by immunofluorescent method with antibodies to andALPHA;-smooth muscle actin and desmin. HCSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCSMC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

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ABC-TC4114

Rat Colonic Smooth Muscle Cells

Rat Colonic Smooth Muscle Cells from Gentaur are isolated from tissue of Sprague-Dawley Rats. Rat Colonic Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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CP-M044

Mouse Colonic Smooth Muscle Cells

Mouse

52538 €

T4079

Human Primary Prostate Smooth Muscle Cells

1053.5 €

T4013

Human Primary Tracheal Smooth Muscle Cells

1053.5 €

ABC-TC3859

Human Umbillical Artery Smooth Muscle Cells

Vascular smooth muscle cells are the cellular substrate of most significant arterial disease. The increased growth potential of vascular smooth muscle cells represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that vascular smooth muscle cells express ICAM-1 and VCAM-1, which may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease. In vitro culture of human VSMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases.HUASMC from Gentaur Research Laboratories are isolated from human umbilical arteries. HUASMC are cryopreserved at the end of secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HUASMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HUASMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUASMC are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

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